Monday, May 23, 2011

THEM2 Antibody

Catalog# :5257

THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several tissues such as heart, kidney, liver and brain and has been shown to be co-localized with beta-tubulin on microtubules. THEM2 interacts with StarD, a protein that plays a key role in fatty acid metabolism, and the addition of StarD to THEM2 increased its catalytic activity, suggesting that THEM2 plays a significant role in the metabolism of fatty acids. At least two isoforms of THEM2 are known to exist.

Additional Names : THEM2, Thioesterase superfamily member 2, Acyl-CoA thioesterase 13, ACOT13, PNAS-27


Western blot analysis of THEM2 in HepG2 cell lysate with THEM2 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Below:Immunohistochemistry of THEM2 in human liver tissue with THEM2 antibody at 2.5 μg/ml.

Other Product Images

Source :THEM2 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human THEM2.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : THEM2 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : THEM2 antibody can be used for detection of THEM2 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5257P - THEM2 Peptide
Long-Term Storage : THEM2 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1211 - HepG2 Cell Lysate
Species Reactivity : H, M, R
GI Number : 8923812
Accession Number : NP_060943
Short Description : Thioesterase superfamily member 2
1. Cheng Z, Song F, Shan X, et al. Crystal structure of human thioesterase superfamily member 2. Biochem. Biophys. Res. Commun. 2006; 349:172-7.
2. Wei J, Kang HW, and Cohen DE. Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs. Biochem. J. 2009; 421:311-22.
3. Cheng Z, Bao S, Shan X, et al. Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule. Biochem. Biophys. Res. Commun. 2006; 350:850-3.
4. Scappa EF, Pocai A, Wu MK, et al. Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD. FASEB J. 2008; 22:2579-90.

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