Monday, May 2, 2011

PARL Antibody

Catalog# :4761

PARL is a mitochondrial integral membrane protein and a member of the highly conserved rhomboid superfamily of membrane proteins. PARL is required for the control of apoptosis during postnatal growth and is required for the processing of OPA1, a protein that prevents the release of cytochrome C from the mitochondria in response to apoptotic signals. In lymphocytes and neurons, PARL’s association with other proteins, such as the Bcl-2 family-related protein Hax1 and Omi can lead to the proteolytic processing of Omi by PARL, preventing the accumulation of Bax, thereby suppressing apoptosis. Variations in this gene have been associated with increased risk for type 2 diabetes. PARL may also play a role in the progression of Alzheimer’s disease through its associations with presenilin-1 and -2. Despite its predicted molecular weight of ~42kDa, PARL is observed at a higher molecular weight in SDS-PAGE. Multiple isoforms of PARL are known to exist.

Additional Names : PARL, Presenilin-associated rhomboid-like protein, PSARL, PSARL1, PSNENIP2


Left: Western blot analysis of PARL in 3T3 cell lysate with PARL antibody at (A) 1 and (B) 2 µg/ml.

Below:Immunocytochemistry of PARL in 3T3 cells with PARL antibody at 2.5 μg/ml.

Other Product Images

Source :PARL antibody was raised against a 15 amino acid peptide from near the amino terminus of human PARL.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : PARL antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies
Application : PARL antibody can be used for detection of PARL by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4761P - PARL Peptide
Long-Term Storage : PARL antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Species Reactivity : H, M, R
GI Number : 143811433
Accession Number : Q9H300
Short Description : Presenilin-associated rhomboid-like protein
1. Pellegrini L, Passer BJ, Canelles M, et al. PAMP and PARL, two novel putative metalloproteases interacting with the COOH-terminus of Presenilin-1 and -2. J. Alzheimers Dis. 2001; 3:181-90.
2. Cipolat S, Rudka T, Hartmann D, et al. Mitochondrial rhomboid PARL regulates cytochrome c release during apoptosis via OPA1-dependent cristae remodeling. Cell 2006; 126:163-75.
3. Chao JR, Parganas E, Boyd K, et al. Hax1-mediated processing of HtrA2 by PARL allows survival of lymphocytes and neurons. Nature 2008; 452:98-102.
4. Walder K, Kerr-Bayles L, Civitarese A, et al. The mitochondrial rhomboid protease PSARL is a new candidate gene for type 2 diabetes. Diabetologia 2005; 48:459-68.

1 comment:

Interleukin said...

Hi friends,

PARL is a protein that in humans is encoded by the PARL gene. It is a member of the rhomboid family of intramembrane serine proteases. This gene encodes a mitochondrial integral membrane protein. Thanks for sharing it......