Tuesday, May 17, 2011

SYPL1 Antibody

Catalog# :5285

The synaptophysin-like protein 1, also known as pantophysin, is a homolog of the neuroendocrine-specific protein synaptophysin, with the highest level of homology across its four transmembrane domains. Unlike synaptophysin however, SYPL1 is ubiquitously expressed and found in small cytoplasmic transport vesicles regardless of their content. SYPL1 is thought to play a multifunctional role in vesicle biogenesis and transport and is a component of adipocyte transport vesicles, and thus may be involved in adipocyte secretion. SYPL1 also interacts with vesicle-associated membrane protein 2 (VAMP-2) in adipocytes and associates with GLUT4-containing vesicles. This antibody will not recognize synaptophysin of SYPL2. Two isoforms of SYPL1 are known to exist; this antibody will only recognize the longer isoform.

Additional Names : SYPL1,Synaptophysin-like 1 protein, H-SP1, pantophysin


Left: Western blot analysis of SYPL1 in human brain tissue lysate with SYPL1 antibody at 1 µg/ml in (A) the absence and (B) the presence of blocking peptide.

Source :SYPL1 antibody was raised against a 16 amino acid peptide from near the amino terminus of human SYPL1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : Polyclonal
Host : SYPL1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : SYPL1 antibody can be used for detection of SYPL1 by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5285P - SYPL1 Peptide
Long-Term Storage : SYPL1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 5803185
Accession Number : NP_006745
Short Description : Synaptophysin-like 1 protein
1. Haass NK, Kartenbeck MA, and Leube RE. Pantophysin is a ubiquitously expressed synaptophysin homologue and defines constitutive transport vesicles. J. Cell Biol. 1996; 134:731-46.
2. Brooks CC, Scherer PE, Cleveland K, et al. Pantophysin is a phosphoprotein component of adipocyte transport vesicles and associates with GLUT4-containin vesicles. J. Biol. Chem. 2000; 275:2029-36.
3. Bradley RL, Cleveland KA, and Cheatham B. The adipocyte as a secretory organ: mechanism of vesicle transport and secretory pathways. Recent Prog. Horm. Res. 2001; 56:329-58.

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