Tuesday, May 24, 2011

beta-Galactosidase Antibody

Catalog# :5155

Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection.

Additional Names : beta-Galactosidase,beta-galactosidase, beta-gal

Left: Western blot analysis of (A) 5 and (B) 25 ng of b-gal with b-gal antibody at 1 µg/ml.

Source :beta-gal antibody was raised against a 17 amino acid peptide from near the amino terminus of E. coli beta-gal.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : beta-Galactosidase antibody was raised in chicken.
Please use anti-chicken secondary antibodies.
Application : beta-gal antibody can be used for detection of beta-gal by Western blot at 1 - 2 µg/ml.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 5155P - beta-Galactosidase Peptide
Long-Term Storage : beta-Galactosidase antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity : Bc
GI Number : 26246355
Accession Number : NP_752394
Short Description : Beta-galactosidase
1. Matthews BW. The structure of E. coli beta-galactosidase. C. R. Biol. 2005; 328:549-56

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