Actin reorganization is important for the regulation of neuronal morphology. A protein involved in this process, the transducer of cdc42-dependent actin assembly 1 (TOCA-1) protein, a member of the evolutionarily conserved pombe CDC15 homology (PCH) protein family, is an essential component of the Cdc42 pathway. TOCA-1 binds both N-WASP and Cdc42 and is essential for Cdc42- and PIP2-induced actin polymerization, suggesting that TOCA-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Decreased expression of TOCA-1 significantly enhances neurite elongation in PC-12 cells; its overexpression in the same cells suppresses neurite elongation. It has been suggested that TOCA-1 negatively regulates axon branching by regulating membrane trafficking by regulating membrane trafficking through the F-BAR/EFC domain. Multiple isoforms of TOCA-1 are known to exist.
Additional Names : TOCA-1 (NT), Transducer of cdc42-dependent actin assembly 1, formin binding protein 1-like, FNBP1L
Left Western blot analysis of TOCA-1 in human brain tissue lysate in (A) the absence and (B) the presence of blocking peptide with TOCA-1 antibody at 0.5 µg/ml.
Below Immunohistochemical staining of human brain tissue using TOCA-1 antibody at 2.5 µg/ml.
Other Product Images
Source :TOCA-1 antibody was raised against a 15 amino acid peptide near the amino terminus of the human TOCA-1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : TOCA-1 antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
Application : TOCA-1 antibody can be used for detection of TOCA-1 by Western blot at 0.5 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 4375P - TOCA-1 Peptide
Long-Term Storage : TOCA-1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1303 - Human Brain Tissue Lysate
Species Reactivity : H, M, R
GI Number : 68348709
Accession Number : NP_001020119
Short Description : (NT) Transducer of cdc42-dependent actin assembly 1
1. Ho H-Y H, Rohatgi R, Lebensohn AM, et al. Toca-1 mediates Cdc42-dependent actin nucleation by activating the N-WASP-WIP complex. Cell 2004; 118:203-16.
2. Kakimoto T, Katoh H, and Negishi M. Regulation of neuronal morphology by Toca-1, an F-BAR/EFC protein that induces plasma membrane invagination. J. Biol. Chem. 2006; 281:29042-43.