Although initially described as an adipocyte-specific triacylglycerol lipase, lipe (also known as hormone-specific lipase or HSL) is expressed in multiple tissues and cell lines. It plays multiple roles in lipid metabolism, including hormone-stimulated lipolysis in adipose tissue and the hydrolysis of cholesterol esters. Lipe is expressed as a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesterol esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. Recently, it was observed that the lack of lipe in genetically obese leptin-null mice inhibited obesity and adipogenesis, suggesting that lipe plays a major role in adipocyte proliferation.
Source :Lipe antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human lipe.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Lipe antibody was raised in rabbit.
Application : Lipe antibody can be used for detection of lipe by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide :Cat.No. 3965P - Lipe Peptide
Long-Term Storage : Lipe antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control :
1. Cat. No. 1369 - Human Lymph node Tissue Lysate
Species Reactivity : H, M, R
GI Number : 896474
Accession Number : ABA03168
Short Description : hormone-sensitive lipase
1. Yeaman SJ. Hormone-sensitive lipase – new roles for an old enzyme. Biochem. J. 2004; 379:11-22.
2. Holst LS, Langin D, Mulder H, et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics 1996; 35:441-7.
3. Kraemer FB, Shen WJ, Harada K, et al. Hormone-sensitive lipase is required for high-density lipoprotein cholesterol ester-supported adrenal steroidogenesis. Mol. Endocrinol. 2004; 18:549-57.
4. Sekiya M, Osuga J, Okazaki H, et al. Absence of hormone-sensitive lipase inhibits obesity and adipogenesis in Lep ob/ob mice. J. Biol. Chem. 2004; 279:15084-90.