Wednesday, September 15, 2010

Adenovirus-9 E4 Orf1 Antibody

Adenovirus-9 E4 Orf1 Antibody

Catalog# : 4749

The many different serotypes of human adenoviruses (Ad) are divided into six subgroups, of which all Ad subgroup A and B and two subgroup D Ads can elicit tumors in infected rodents. Unlike the Ads from subgroup A and B, the ones from subgroup D, Ad9 and Ad10 elicit estrogen-dependent mammary tumors as opposed to undifferentiated sarcomas. In the case of Ad9, its tumorigenicity is dependent on the product of the open reading frame (ORF) 1 of the early region 4 (E4). The tumorigenic potential of Ad9 E4 Orf1 depends on a carboxyl-terminal PDZ domain-binding motif that mediates interactions with several different membrane-associated cellular proteins such as MUPP1, PATJ, MAGI-1, ZO-2 and Dlg1. It has been suggested that Ad9 E4 Orf1 may have evolved from an ancestral cellular dUTP pyrophosphatase.
Additional Names : Adenovirus-9 E4 Orf1, Ad-9 E4 Orf1

Source : Ad-9 E4 Orf1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of the Ad-9 E4 Orf1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Adenovirus-9 E4 Orf1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : Ad-9 E4 Orf1 antibody can detect 10ng Ad-9 E4 Orf1 peptide in ELISA at 1 µg/ml.
Tested Application(s) : E
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4749P - Adenovirus-9 E4 Orf1 Peptide
Long-Term Storage : Adenovirus-9 E4 Orf1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity :V
GI Number : 62510674
Accession Number : P89079
Short Description : Open reading frame 1 of the E4 region of Adenovirus 9

1. Shenk T. Adenoviridae: the viruses and their replication, p. 2265-2300 in DM Knipe and PM Howley (ed.) Fields virology, 4th ed. Vol. 2. 2001. Lippincott-Raven Publishers, Philadelphia, PA.
2. Javier R, Rasha Jr. K, Macdonald GJ, et al. Human adenovirus type 9-induced rat mammary tumors. J. Virol. 1991; 65:3192-202.
3. Javier RT. Adenovirus type 9 E4 open reading frame 1 encodes a transforming protein required for the production of mammary tumors in rats. J. Virol. 1994; 68:3917-24.
4. Weiss RS, Lee SS, Prasad BV, et al. Human adenovirus early region 4 open reading frame 1 genes encode growth-transforming proteins that may be distantly related to dUTP pyrophosphatase enzymes. 1997; J. Virol. 1997; 71:1857-70.

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