A branching monomer is required to construct comb-like oligonucleotide probes. The Symmetric Branching generates two identical branches at the 5' terminus of an oligonucleotide during automated DNA synthesis. The number of branches added increases exponentially with each addition of the branching modifier. The Asymmetric Branching modifier generates two asymmetric branches at the 5' terminus of oligonucleotide during automated DNA synthesis. Multiple additions of this phosphoramidite generate "comb" type oligonucleotide structures.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
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