Monday, September 20, 2010

CEP164 Antibody

CEP164 Antibody

Catalog# : 5083

CEP164 was initially identified as a centrosomal protein, but other studies have indicated that it also plays a role in the formation of primary cilia, the microtubule-based sensory antennae projecting from the surface of many eukaryotic cells as well as in DNA damage response acting as a mediator protein. CEP164 interacts with both ATR and ATM, proteins that trigger a number of cellular responses including the initiation of DNA damaged-induced cell cycle checkpoints. It is phosphorylated upon replication stress, ultraviolet (UV) radiation, and ionizing radiation; silencing of CEP164 significantly reduces the DNA damage-induced phosphorylation of several proteins in the DNA damage-activated signaling cascade and compromises cell survival after UV damage. At least two isoforms of CEP164 are known to exist.

Additional Names : CEP164, Centrosomal protein 164
Left: Western blot analysis of CEP164 in rat heart tissue lysate with CEP164 antibody at (A) 1 and (B) 2 µg/ml.

Below: Immunohistochemistry of CEP164 in mouse heart tissue with CEP164 antibody at 5 µg/ml.

Other Product Images

Source : CEP164 antibody was raised against an 18 amino acid peptide near the amino terminus of human CEP164.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CEP164 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CEP164 antibody can be used for detection of CEP164 by Western blot at 1 – 2 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 5083P - CEP164 Peptide
Long-Term Storage : CEP164 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1461 - Rat Heart Tissue Lysate
Species Reactivity :H, M, R
GI Number : 115648142
Accession Number : NP_055771
Short Description : Centrosomal protein 164
1. Andersen JS, Wilkinson CJ, Mayor T, et al. Proteomic characterization of the human centrosome by protein correlation profiling. Nature 2003; 426:570-4.
2. Graser S, Stierhof YD, Lavoie SB, et al. Cep164, a novel centriole appendage protein required for primary cilium formation. J. Cell Biol. 2007; 179:321-30.
3. Sivasubramaniam S, Sun X, Pan YR, et al. Cep164 is a mediator protein required for the maintenance of genomic stability through modulation of MDC1, RPA, and CHK1. Genes Dev. 2008; 22:587-600.
4. Pan Y-R and Lee EY-HP. UV-dependent interaction between Cep164 and XPA mediates localization of Cep164 at sites of DNA damage and UV sensitivity. Cell Cycle 2009; 8:655-64.

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