Tuesday, September 28, 2010

CCR8 Antibody

CCR8 Antibody

Catalog# : 2097

CCR8 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human and murine CCR8 were cloned and designated TER1, CKR-L1, and ChemR1. The encoded seven transmembrane protein was identified as the receptor for human CC chemokine I-309 and renamed CCR8. Recently, CCR8 was found to serve as a coreceptor for diverse T-cell tropic, dual-tropic and macrophage-tropic HIV-1 strains. CCR8 mediates CC chemokine I-309 induced monocyte chemoattraction and HIV-1 envelope fusion and virus infection, which can be prevented by the CCR8 ligand I-309. CCR8 is expressed in spleen, thymus and T lymphoblastic cell lines.

Additional Names : CCR8 (EL), CCR8
Left: Western blot analysis of CCR8 in human spleen lysate with CCR8 antibody at 1:500 dilution.

Source : CCR8 antibody was raised against a peptide corresponding to amino acids 183 to 201 of human CCR8, which locate in the second extracellular loop.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CCR8 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CCR8 (Extracellular Loop) Peptide (Cat. No. 2097P)
Application : CCR8 antibody can be used for detection of CCR8 by Western blot at 1:500 to 1:1000 dilution. An approximately 50 kDa band can be detected.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2097P - CCR8 Peptide
Long-Term Storage : CCR8 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate
Species Reactivity :H
GI Number : 2231165
Accession Number : U45983
Short Description : (EL) HIV & chemokine receptor
1. Napolitano M, Zingoni A, Bernardini G, Spinetti G, Nista A, Storlazzi CT, Rocchi M, Santoni A. Molecular cloning of TER1, a chemokine receptor-like gene expressed by lymphoid tissues. J Immunol 1996;157:2759-63
2. Zaballos A, Varona R, Gutierrez J, Lind P, Marquez G. Molecular cloning and RNA expression of two new human chemokine receptor-like genes. Biochem Biophys Res Commun 1996;227:846-53
3. Samson M, Stordeur P, Labbe O, Soularue P, Vassart G, Parmentier M. Molecular cloning and chromosomal mapping of a novel human gene, ChemR1, expressed in T lymphocytes and polymorphonuclear cells and encoding a putative chemokine receptor. Eur J Immunol 1996;26:3021-8
4. Goya I, Gutierrez J, Varona R, Kremer L, Zaballos A, Marquez G. Identification of CCR8 as the specific receptor for the human beta-chemokine I-309: cloning and molecular characterization of murine CCR8 as the receptor for TCA-3. J Immunol 1998;160:1975-81

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