Catalog# : 1112
Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The b-chemokines RANTES, MIP-1alpha and MIP-1beta are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.
Additional Names : CCR5 (NT), CCR5
Left: Western blot analysis of CCR5 in THP-1 whole cell lysate with CCR5 antibody at 1:1000 dilution.
Below: Immunohistochemistry of CCR5 in human lymph node tissue with CCR5 antibody at 20 µg/ml.
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Source : CCR5 antibody was raised against a peptide corresponding to amino acids 6 to 20 of human CCR5 .
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : CCR5 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CCR5 (N-Terminus) Peptide (Cat. No. 1112P)
Application : CCR5 antibody can be used for Western blot at 1:1000 to 1:2000 dilution. An approximate 40 kDa band can be detected. CCR5 antibody can also detect CCR5 by immunohistochemistry at 20 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1112P - CCR5 Peptide
Long-Term Storage : CCR5 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1. Cat. No. 1208 - THP-1 Cell Lysate
2. Cat. No. 1369 - Human Lymph Node Tissue Lysate
Species Reactivity :H
GI Number : 2104520
Accession Number : AAB57793
Short Description : (NT) HIV & chemokine receptor
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2. Littman, DR. Cell 1998;93:677-80
3. Deng, H, et al. Nature 1996;381:661-6
4. Dragic, T, et al. Nature 1996;381:667-73