Sunday, September 12, 2010

Avian Influenza Neuraminidase Antibody

Avian Influenza Neuraminidase Antibody

Catalog# : 3423

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1). Novel influenza virus strains emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found (2). These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong (3). There was some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low (4). Although it has been known that cleavage site and glycosylation patterns of the HA protein play important roles in determining the pathogenicity of H5 avian influenza viruses, it has only recently been shown that an additional glycosylation site within the globular head of the NA protein also contributes to the high virulence of the H5N1 virus (5).
Additional Names : Avian Influenza Neuraminidase (CT), Neuraminidase

Source : Avian influenza neuraminidase antibody was raised against a synthetic peptide corresponding to 15 amino acids at the carboxy terminus of the avian influenza neuraminidase protein. Efforts were made to use relatively conserved regions as the antigen.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Avian Influenza Neuraminidase antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Avian Influenza Neuriminidase (C-Terminus) Peptide (Cat. No. 3423P)
Application : Avian influenza neuriminidase antibody can be used for the detection of the avian influenza neuriminidase protein from the H5N1 strain of Avian influenza A in ELISA. It will detect 10 ng of free peptide at 1 µg/ml.Other applications are pending.
Tested Application(s) : E
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 3423P - Neuriminidase Peptide
Long-Term Storage : Avian Influenza Neuraminidase antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity :V
GI Number : 39840718
Accession Number : CAC95053
Short Description : (CT) Avian Flu H5N1 Neuraminidase
1. Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-186.
2. Alexander DJ. A review of avian influenza. Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on Influenza Viruses of Wild and Domestic Animals. Vet. Microbiol. 2000; 74:3-13.
3. Shortridge KF, Zhou NN, Guan Y, et al. Characterization of avian H5N1 influenza viruses from poultry in Hong Kong. Virol. 1998; 252:331-342.
4. Buxton Bridges C, Katz JM, Seto WH, et al. Risk of influenza A (H5N1) infection among health care workers exposed to patients with influenza A (H5N1), Hong Kong. J. Inf. Dis. 2000; 181:344-8.

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