Catalog# : 2067
Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase . ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues .
Additional Names : ZIPK (IN), ZIPK
Left: Western blot analysis of ZIP kinase in HeLa (H) and Jurkat (J) whole cell lysates with ZIP kinase antibody at 1 µg/ml.
Below: Immunocytochemistry of ZIP kinase in Jurkat cells with ZIP kinase antibody at 10 µg/ml.
Other Product Images
Source : ZIP kinase antibody was raised against a peptide corresponding to amino acids near the center of human ZIP kinase.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : ZIPK antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human ZIPK (Intermediate Domain) Peptide (Cat. No. 2067P)
Application : ZIPK antibody can be used for detection of DNase II expression by Western blot at 1:500 to 1:1000 dilution. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II.
Tested Application(s) : E, WB, ICC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 2067P - ZIPK Peptide
Long-Term Storage : ZIPK antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
1. Cat. No. 1306 - Human Spleen Tissue Lysate
2. Cat. No. 1208 - THP-1 Whole Cell Lysate
Species Reactivity :H, M, R
GI Number : 5162884
Accession Number : BAA81746
Short Description : (IN) Apoptosis inducing kinase
1. Kawai T, Matsumoto M, Takeda K, Sanjo H, Akira S. ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. Mol Cell Biol 1998;18:1642-51 (RD1299)