Monday, September 20, 2010

CUEDC1 Antibody

CUEDC1 Antibody

Catalog# : 4829

The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC1 (CUE domain-containing protein 1) is a 386 amino acid protein that contains one CUE domain, suggesting a possible role in protein trafficking and degradation pathways. Two isoforms of CUEDC1 exist due to alternative splicing events. Defects in the gene encoding CUEDC1 may be associated with early stage cervical cancer, implicating CUEDC1 as a potential tumor marker. CUEDC1 antibody will not cross-react with CUEDC2.

Additional Names : CUEDC1, CUE Domain-Containing Protein 1
Left: Western blot analysis of CUEDC1 in rat liver tissue lysate with CUEDC1 antibody at (A) 0.5 and (B) 1 µg/ml.

Below: Immunohistochemistry of CUEDC1 in human liver tissue with CUEDC1 antibody at 2 µg/ml.

Other Product Images

Source : CUEDC1 antibody was raised against a 16 amino acid peptide near the carbocy terminus of human CUEDC1.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : CUEDC1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : CUEDC1 antibody can be used for detection of CUEDC1 by Western blot at 0.5 – 1 µg/ml.
Tested Application(s) : E, WB, IHC
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 4829P - CUEDC1 Peptide
Long-Term Storage : CUEDC1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1464 - Rat Liver Tissue Lysate
Species Reactivity :H, M, R
GI Number : 34785153
Accession Number : AAH56882
Short Description : CUE Domain-Containing Protein 1
1. Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J. 2003; 22:1273-81.
2. Ponting CP. Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction. Biochem. J. 2000; 351:527-35.
3. Prag G, Misra S, Jones EA, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell 2003; 113:609-20.
4. Kang RS, Daniels CM, Francis SA, et al. Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding. Cell 2003; 113:621-30.

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