Sunday, September 12, 2010

Avian Influenza Nonstructural Protein 1 Antibody

Avian Influenza Nonstructural Protein 1 Antibody

Catalog# : 3915

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however, it is in birds that all subtypes, including the so-called “avian flu” or H5N1, can be found. These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. One of the less studied proteins encoded by, but not incorporated in, the influenza virus is the nonstructural protein (NS) 1. NS1 counters cellular antiviral activities and acts as a virulence factor. It can bind to double-stranded RNA and sequester it from 2’-5’ OAS, preventing the activation of the RNAse L, which normally acts to degrade RNA and prevent virus replication. NS1 also binds to and inhibits the anti-viral protein kinase PKR.
Additional Names : Avian Influenza Nonstructural Protein 1, H5N1 NS1, Nonstructural protein 1

Source : Avian Influenza Nonstructural Protein 1 antibody was raised against a synthetic peptide corresponding to 14 amino acids at the carboxy terminus of the Avian Influenza Nonstructural Protein 1 protein. Efforts were made to use relatively conserved regions of the viral sequence as the antigen.
Purification : Affinity chromatography purified via peptide column
Clonality and Clone : This is a polyclonal antibody.
Host : Avian Influenza Nonstructural Protein 1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Application : Avian Influenza Nonstructural Protein 1 antibody can be used for the detection of the avian influenza nonstructural protein 1 protein from influenza A in ELISA.
Tested Application(s) : E
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat.No. 3915P - Avian Influenza Nonstructural Protein 1 Peptide
Long-Term Storage : Avian Influenza Nonstructural Protein 1 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Species Reactivity :V
GI Number : 85680915
Accession Number : ABC72653
Short Description : H5N1, virulence factor, dsRNA, RNase L
1. Thompson WW, Shay DK, Weintraub, et al. Mortality associated with influenza and respiratory syncytial virus in the United States. JAMA 2003; 289:179-186.
2. Alexander DJ. A review of avian influenza. Proceedings of the European Society for Veterinary Virology (ESVV) Symposium on Influenza Viruses of Wild and Domestic Animals. Vet. Microbiol. 2000; 74:3-13.
3. Krug RM, Yuan W, Noah D, et al. Intracellular warfare between human influenza viruses and human cells: the role of the viral NS1 protein. Virology 2003; 309:181-9.
4. Min J-Y and Krug RM. The primary function of RNA binding by the influenza A virus NS1 protein is infected cells: inhibiting the 2’-5’ oligo (A) synthase/RNase L pathway. Proc. Natl. Acad. Sci. USA 2006; 103:7100-5.

No comments: