Monday, September 6, 2010

XAF-1 Antibody

XAF-1 Antibody

Catalog# : 3409

XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and –9, and triggers its relocation from the cytosol to the nucleus (1,2). Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death (1). XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines (3). In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines (2,3). Both IFN-alpha2 and IFN-beta can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs (4).

Additional Names : XAF-1 (IN), XAF-1
Left: Western blot analysis of XAF-1 in human spleen lysate with XAF-1 antibody (IN) at 1 (lane A), 2 (lane B), and 4 (lane C) µg/ml, respectively.

Source : XAF-1 antibody was raised against a synthetic peptide corresponding to 16 amino acids near the middle of human XAF-1.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : XAF-1 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Immunogen : Human XAF-1 (Intermediate Domain) Peptide (Cat. No. 3409P)

Application : XAF-1 antibody can be used for the detection of XAF-1 by Western blot at 1-2 µg/ml.Multiple isoforms of XAF-1 exist and a doublet at ~30 kDa can be seen using anti-XAF-1 (IN).

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat. No. 3409P - XAF-1 Peptide

Long-Term Storage : XAF-1antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1306 - Human Spleen Tissue Lysate

Species Reactivity :H

GI Number : 1869901

Accession Number : CAA68030

Short Description : (IN) Inhibitor of XIAP

1. Liston P, Fong W, Kelly NL, et al. Identification of XAF1 as an antagonist of XIAP anticaspase activity. Nature Cell Biol. 2001;3:128-33.
2. Deveraux QL, Takahashi R, Savesan GS, and Reed JC. X-linked IAP is a direct inhibitor of cell-death proteases. Nature 1997;388:300-4.
3. Fong WG, Liston P, Rajcan-Separovic E, et al. Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics 2000;70:113-122.
4. Leaman DW, Chawla-Sarkar M, Vyas K, et al. Identification of X-linked inhibitor of apoptosis associated factor-1 as an interferon-stimulated gene that augments TRAIL/Apo2L-induced apoptosis. J. Biol. Chem. 2002;277:28504-11.

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