Sunday, September 26, 2010

Protein Carrier Conjugation

Bio-Synthesis offers conjugation of peptides to a variety of immunogenic carrier proteins, and gel, for use as an immunogen or for affinity purification. A variety of immunogenic carrier proteins such as Keyhole Limpet Hemocyanin (KLH) and Diphtheria toxoid have been use in conjugation with several conjugation chemistries. We also offer coupling of peptides to various types of gel to create an immunosorbent, for positive or negative selection of macromolecular binding entries, such as antibodies.

Serum production packages also available with large selection of host animals, click here to explore more on our custom peptide antibody production.

Carrier: Keyhole Limpet Hemocyanin (KLH), Bovine Serum Albumin (BSA), Rabbit Serum Albumin (RSA), Ovalbumin, Thyroglobulin, or Human Gamma Globulin (HGG). Other proteins are available upon request.

Cross-Links: MBS (and other bi-functional linkers), which couple through sulfhydryl groups, and glutaraldehyde, which couples primarily through alpha, sigma & epsilon amino groups

* Amino Acid Analysis of Conjugates available upon request

[1] Lee, A.C., Powell, J.E., Tregear, G.W., Niall, H.D. and Stevens, V.C. (1980) A method for preparing b-hCG COOH peptide-carrier conjugates of predictable composition. Mol. Immunol. 17; 749-756.

[2] Posnett, D., McGrath, H., and Tam, J.P. A novel method for producing anti-peptide antibodies
, The Journal of Biological Chemistry, 263, 1719-1725 (1988),

[3] Tam, J.P., Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system, Proc. Natl. Acad, Sci., 85, 5409-5413 (1988).

[4] Wang, C. Y., Looney, D.J., Li, M.L., Walfield, A.M., Ye, J., Hosein, B., Tam, J.P. and Wong-Staal, F., Long-term high-titer neutralizing activity induced by octameric synthetic HIV antigen, Science, 254, 285-288. (1991)

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