Carboxy dT Modifier
Carboxy-dT can be used in place of a dT in an internal position of the oligo, or as the 5' terminal base. The carboxy-dT modifier allows direct coupling of an oligo to a molecule or surface with a primary amino by a standard peptide coupling or via the intermediate N-hydroxy-succinimide (NHS) ester. This modifier is added to the oligo on the machine during synthesis.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
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