Wednesday, September 29, 2010

CXCR4 Antibody

CXCR4 Antibody

Catalog# : 1012

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells. CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2. The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1. CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells. CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
Additional Names : CXCR4 (EL), CXCR4

Left: Western blot analysis of CXCR4 in HeLa total cell lysate with anti-CXCR4 (EL) at 1:1000 dilution.

Source : CXCR4 antibody was raised against a peptide corresponding to amino acids 182 to 196 in the second extracellular loop (EL) of human CXCR4.
Purification : Antibody is DEAE purified
Clonality and Clone : This is a polyclonal antibody.
Host : CXCR4 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.
Immunogen : Human CXCR4 (Extracellular Loop) Peptide (Cat. No. 1012P)
Application : CXCR4 antibody can be used for Western blot at 1:500 to 1:1000 dilution.
Tested Application(s) : E, WB
Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide : Cat. No. 1012P - CXCR4 Peptide
Long-Term Storage : CXCR4 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
1. Cat. No. 1201 - HeLa Cell Lysate
Species Reactivity :H, M
GI Number : 4503175
Accession Number : NP_003458
Short Description : (EL) HIV & chemokine receptor
1. Dimitrov DS. Cell 1997;91:721-730
2. Feng Y et al. Science 1996;272:872-7
3. Berson JF et al. J Virol 1996;70:6288-95
4. Doranz BJ et al. Cell 1996;85:1149-1158

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