Wednesday, September 1, 2010
A primary amino group can be used to attach a variety of modifiers (such as fluorescent dyes) to an oligonucleotide or used to attach an oligonucleotide to a solid surface, for example, a primary amine (NH2) at at the 5’-position is used to functionalize the corresponding terminus of the nucleotide for conjugation with e.g. an activated NHS ester or isothiocyanate fluorescent label. The 3'-NH2 modification allows the prevention of the 3' to 5' exonuclease degradation of oligonucleotides in biological fluids such as cell culture medium.
C6, C12 methylene (CH2) or longer and all of them are hydrophobic. Uni-LinkTM Amino Modifier provides a free primary amine attached to the 5'-end of an oligo via a six carbon aliphatic spacer arm. It is functionally interchangeable with
Amino Modifier C6 Amino-Modifier dA, Amino-Modifier dC, Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis.
Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.
Minimum Guarantee Purified Yield
Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.
Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.
For additional information, please send your request to firstname.lastname@example.org or call us at 1-800-227-0627.