Wednesday, September 1, 2010

SPG15 Antibody

SPG15 Antibody

Catalog# : 5023

Hereditary spastic paraplegias (HSPs) are genetically and phenotypically heterogeneous disorders. Spastic paraplegia with thinning of the corpus callosum (ARHSP-TCC) is a relatively frequent form of complicated hereditary spastic paraplegia in which mental retardation and muscle stiffness at onset are followed by slowly progressive paraparesis and cognitive deterioration. SPG15 is the second gene known to be responsible for ARHSP-TCC in the Italian population. Mutations in this gene are associated with autosomal recessive spastic paraplegia-15. SPG15 encodes a protein containing a FYVE zinc finger binding domain which is thought to target these proteins to membrane lipids through interaction with phospholipids in the membrane. SPG15 mRNA is widely distributed in human tissues, as well as in rat embryos, suggesting a possible role for this protein during embryonic development. SPG15 co-localizes partially with endoplasmic reticulum and endosome markers, suggesting a role in intracellular trafficking. Multiple isoforms of SPG15 are known to exist.

Additional Names : SPG15, Autosomal recessive spastic paraplegia 15, ZFYVE26

Left: Western blot analysis of SPG15 in rat heart tissue lysate with SPG15 antibody at (A) 0.5 and (B) 1 µg/ml.

Source : SPG15 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human SPG15.

Purification : Affinity chromatography purified via peptide column

Clonality and Clone : This is a polyclonal antibody.

Host : SPG15 antibody was raised in rabbit. Please use anti-rabbit secondary antibodies.

Application : SPG15 antibody can be used for detection of SPG15 by Western blot at 0.5 – 1 µg/ml.

Tested Application(s) : E, WB

Buffer : Antibody is supplied in PBS containing 0.02% sodium azide.

Blocking Peptide : Cat.No. 5023P - SPG15 Peptide

Long-Term Storage : SPG15 antibody can be stored at 4ºC, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Positive Control
1. Cat. No. 1461 - Rat Heart Tissue Lysate

Species Reactivity :H, M, R

GI Number : 119601358

Accession Number : EAW80952

Short Description : Programmed cell death protein 5

1. Hughes CA, Byrne PC, Webb S, et al. SPG15, a new locus for autosomal recessive complicated HSP on chromosome 14q. Neurology 2001; 56:1230-3.
2. Denora PS, Muglia M, Casali C, et al. Spastic paraplegia with thinning of the corpus callosum and white matter abnormalities: further mutations and relative frequency in ZFYVE26/SPG15 in the Italian population. J. Neurol. Sci. 2009; 277:22-5.
3. Hanein S, Martin E, Boukhris A, et al. Identification of the SPG15 gene, encoding spastizin, as a frequent cause of complicated autosomal-recessive spastic paraplegia, including Kjellin syndrome. Am. J. Hum. Genet. 2008; 82:992-1002.
4. Boukhris A, Feki I, Denis E, et al. Spastic paraplegia 15: linkage and clinical description of three Tunisian families. Mov. Disord. 2008; 23:429-33.

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